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商品編號:16928

码报最准网站:KK2602 KAPA KAPA 熱啟動HiFi高保真酶 ReadyMix (6.25ml)

品牌:Roche-KAPA
規格:6.25ml
參考價格:2975
剩余數量:00門店價:¥0
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品牌:KAPA,~~~~~~~~~~ 規格:6.25ml~~~~~~~~~~ 價格:2975元~~~~~~~~~~~~~

KAPA Library Amplification Kits contain KAPA HiFi DNA Polymerase, a novel enzyme engineered for ultra-high fidelity and robustness using Kapa’s directed evolution technology platform.

KAPA HiFi has become the enzyme of choice for NGS library amplification due to its ability to amplify complex DNA populations with high fidelity, high efficiency, decreased PCR duplication rates and very low bias.* This results in lower duplication rates and improved coverage of GC- and AT rich regions, promoters, low complexity and other challenging regions in all NGS library construction workflows requiring library amplification.

In addition to the standard library amplification formulation, KAPA HiFi is available in a unique real-time formulation, for applications that demand precise control over library amplification. A uracil-tolerant variant, KAPA HiFi Uracil+ is also available for the high-efficiency, high-fidelity, low-bias amplification of libraries constructed from bisulfite-treated DNA.

KAPA Library Amplification Kits for Illumina® platforms now also include an optimally formulated Library Amplification Primer Mix.

*Data on file.

For Research Use Only. Not for use in diagnostic procedures.

Product Highlights

Achieve the lowest amplification bias and duplication rates

  • Lower amplification bias result in improved representation of all library fragments and sequence regions
  • High-efficiency amplification leads to fewer amplification cycles and lower PCR duplicates
  • Less additional next-generation or Sanger sequencing needed to complete genomes
The first exons of many genes have a high GC content, and are therefore difficult to amplify. Regions of these exons are therefore typically underrepresented in whole exome sequence data. Pre-capture amplification with the evolved, low-bias KAPA HiFi DNA Polymerase results in significantly improved coverage of GC- and AT-rich regions, and other sequence elements that are difficult to amplify. Data courtesy of The Broad Institute. Data on file.

Improve coverage of GC- and AT-rich regions

  • Lower amplification bias improves coverage uniformity of GC- and AT-rich regions, promoters, low complexity and other challenging regions
  • Improved overall coverage and coverage uniformity observed on both Illumina and Ion Torrent™ sequencing platforms
Real-time, high fidelity amplification of multiple libraries with the KAPA Real-Time Library Amplification Kit. Twenty libraries, spanning a ~64-fold concentration range (equivalent to 6 cycles), were simultaneously amplified and terminated after 14 cycles. Fourteen of the twenty libraries fall within the targeted amplification range (the fluorescence range defined by the Real-Time Amplification Standards 1¬–3). The remaining six libraries could either be used as is, noting that they may be outside the optimal concentration range, or they could be re-amplified individually or in high- or low-concentration groups. Data on file.

Exercise precise control over library amplification

  • KAPA Real-Time Amplification Kits allow for library amplification to be observed in real time
  • Terminate amplification at the optimal point for individual samples
  • Optimize library amplification parameters for higher throughput workflows
Error rates of Taq DNA polymerase and polymerases typically used in NGS library amplification. The error rates of proofreading DNA polymerases such as Q5 (New England Biolabs), Phusion (Thermo Scientific) and the evolved KAPA HiFi DNA Polymerase is 50 – 100 times lower than that of Taq. The error rate of KAPA HiFi was confirmed by deep sequencing on the 454 platform, and is 1 error per 3.54 x 106 nucleotides incorporated. Data on file.

Amplify NGS libraries with industry-leading fidelity

  • KAPA HiFi was engineered using directed evolution to have enhanced proofreading (3′-5′ exonuclease) activity
  • Industry-leading fidelity confirmed by 454 sequencing

產品詳情請參閱KAPA官網,鏈接:https://www.kapabiosystems.com/~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 杭州開泰生物技術有限公司上是一家創辦于2006年的創新型高科技企業,自公司成立以來一直專注于為生命科學和生物技術領域的客戶提供優質的產品和技術服務。公司是Tiangen,CST,KAPA,科晶和江蘇MRC的浙江一級代理。同時公司擁有自主研發的漢泰基因品牌高通量測序產品,并提供各種科研技術服務。如需訂購,請在線聯系QQ或者致電:0571-88169527.

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